Journal: Toxins

Article Title: Coagulating Colubrids: Evolutionary, Pathophysiological and Biodiscovery Implications of Venom Variations between Boomslang ( Dispholidus typus ) and Twig Snake ( Thelotornis mossambicanus )

doi: 10.3390/toxins9050171

Figure Lengend Snippet: Snake Venom Metalloprotease Activity of venom (10 ng/µL and 50 ng/µL) was measured based on its ability to cleave a fluorogenic peptide substrate (Mca-PLGL-Dpa-AR-NH2 Fluorogenic MMP Substrate, Cat#ES001). Column graph of Matrix Metalloprotease activity assay of D. typus and T. mossambicanus obtained from normalisation of slope values. X axis: species name and concentration; Y axis: absorbance percentage. Analysis of triplicates was conducted on GraphPad PRISM 7.0 and error bars indicate standard deviation.

Article Snippet: To calculate this, the EC 50 function in GraphPad PRISM 7.0 was used for each data set.

Techniques: Activity Assay, Concentration Assay, Standard Deviation

Journal: Toxins

Article Title: Coagulating Colubrids: Evolutionary, Pathophysiological and Biodiscovery Implications of Venom Variations between Boomslang ( Dispholidus typus ) and Twig Snake ( Thelotornis mossambicanus )

doi: 10.3390/toxins9050171

Figure Lengend Snippet: The kallikrein activity of venom (10 ng/µL and 50 ng/µL) was measured based on its ability to cleave a fluorogenic peptide substrate (Boc-VPR-AMC Fluorogenic Peptide Substrate, Cat#ES011). Column graph of Kallikerin activity assay of D. typus and T. mossambicanus obtained from normalisation of slope values. There is a highly significant difference in activity between D. typus and T. mossambicanus at both concentrations ( p ≤ 0.001). X axis: species name and concentration; Y axis: absorbance as a percentage. Analysis of triplicates was conducted on GraphPad PRISM 7.0 and error bars indicate standard deviation.

Article Snippet: To calculate this, the EC 50 function in GraphPad PRISM 7.0 was used for each data set.

Techniques: Activity Assay, Concentration Assay, Standard Deviation

Journal: Toxins

Article Title: Coagulating Colubrids: Evolutionary, Pathophysiological and Biodiscovery Implications of Venom Variations between Boomslang ( Dispholidus typus ) and Twig Snake ( Thelotornis mossambicanus )

doi: 10.3390/toxins9050171

Figure Lengend Snippet: Secretory Phospholipase A 2 was measured by its ability to cleave a fluorogenic peptide substrate EnzChek ® (Cat# E10217). Column graph of sPLA 2 activity assay of D. typus and T. mossambicanus obtained from normalisation of slope values. There is a highly significant difference in activity between D. typus from the positive control and between D. typus and T. mossambicanus ( p ≤ 0.001). There is also a significant difference of T. mossambicanus from the positive control ( p ≤ 0.01). X axis: species name and concentration; Y axis: slope as a relative percentage. Analysis of triplicates was conducted on GraphPad PRISM 7.0 and error bars indicate standard deviation.

Article Snippet: To calculate this, the EC 50 function in GraphPad PRISM 7.0 was used for each data set.

Techniques: Activity Assay, Positive Control, Concentration Assay, Standard Deviation

Journal: Toxins

Article Title: Coagulating Colubrids: Evolutionary, Pathophysiological and Biodiscovery Implications of Venom Variations between Boomslang ( Dispholidus typus ) and Twig Snake ( Thelotornis mossambicanus )

doi: 10.3390/toxins9050171

Figure Lengend Snippet: Normalisation of transformed data; antivenom and procoagulant effects of D. typus and T. mossambicanus . D. typus clotting curve given in blue circles, AV D. typus = antivenom response curve given in purple circles, T. mossambicanus clotting curve given in red squares, AV T. mossambicanus = antivenom response curve given in green squares. X axis: log concentration, Y axis: Normalised time (%) (for sub data sets). Analysis performed in GraphPad PRISM 7.0. Values are averages of triplicates (single dilution measured three times), and standard deviation error bars are shown for each, although for most the error range is smaller than the line icon.

Article Snippet: To calculate this, the EC 50 function in GraphPad PRISM 7.0 was used for each data set.

Techniques: Transformation Assay, Coagulation, Concentration Assay, Standard Deviation